Journal: Clinical Epigenetics

Article Title: Intra-individual changes in DNA methylation not mediated by cell-type composition are correlated with aging during childhood

doi: 10.1186/s13148-016-0277-3

Figure Lengend Snippet: Chromosomal positions of mean DNA methylation differences. a Modified Manhattan plot of mean DNA differences by chromosomal position along the x -axis and mean DNA methylation differences on the y -axis. Each point represents a CpG site with mean DNA methylation differences between 2 and 10 years ( black ) and between 10 and 16 years ( gray ) for CpGs and mean DNA methylation differences between 2 and 10 years ( red ) and between 10 and 16 years ( green ) at the aDMPs ( n = 26). b Scatter plot of DNA methylation at CpGs situated in a CpG island overlapping the promoter and the first exon of SOCS3 generated by RRBS ( upper panel ) and EpiTYPER (replication data set, bottom panel ) on the y -axis and chromosomal position on the x -axis. Each point represents a CpG per sample colored by age ( red = 2, blue = 10, and green = 16 years) sorted by chromosomal position, and each point is jittered by 0.1). Smoothening lines represents local regression colored by time points. The significant aDMP (chr17: 76355061) is highlighted between vertical lines

Article Snippet: The Sequenom MassARRAY EpiTYPER platform was used to replicate a subset of the aDMPs in a larger set of samples ( n = 31) selected from the same cohort.

Techniques: DNA Methylation Assay, Modification, Generated